Membrane Function Lab Planning – Hour 4, Group 3

Consider the following questions to help you get started:

  • What variable will you be testing (independent variable)?
  • What variable(s) will you be measuring (dependent variable)?
  • What variables will you hold constant (constant variable)?
  • What evidence would confirm that the stain has crossed the membrane?
  • How will you be confident in the validity of your results?
  • What will you use as a standard of comparison (control group)?

Use the comment form below to discuss the plan for your experiment.

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7 Responses to Membrane Function Lab Planning – Hour 4, Group 3

  1. Shruthi V. says:

    Hey guys! For our experiment, I think that first, we should put Congo red in the active yeast cells and see if it is removed from the cell over time through active transport, which would prove our hypotheses. Also, we should do the same thing with the boiled yeast cells to confirm that this change is because of the active transport.

  2. Shruthi V. says:

    We should probably also just look at plain, healthy yeast cells under the microscope for a standard of comparison.

  3. Katherine P says:

    Sounds great. Also, to make sure our results are accurate, we should perform this experiment at least 2-3 times.

  4. Sean D says:

    I agree with everything that has been stated above. In addition to examining the healthy yeast cells prior to adding the dye we should also examine the boiled yeast cells for a reference point before we add the dye.

  5. Olivia Baxter says:

    I agree, that sounds like a good experiment that will prove our hypothesis. Our independent variable would be the amount of dye put into the yeast cells. The dependent variable would be the time it takes for the cells to transport the dye out.

  6. Olivia Baxter and Katherine Poer says:

    First, put one drop of healthy yeast on a slide and cover with cover slip and observe under microscope (control group). Next, put one drop of healthy yeast on another slide and put a drop of Congo red dye over it an mix with toothpick. Then, cover with cover slip and observe under different microscope. Finally, put one drop of boiled yeast cells on a slide and mix a drop of Congo red dye with a toothpick. Observe under microscope and clean up when finished.

  7. Shruthi V. and Sean D. says:

    Through this experiment, we proved our hypotheses correct. The stain moves into the yeast cells through passive transport and then moves out of the yeast cells through active transport. We know this because when we put Congo red dye in the yeast, the cells were red at first, and then gradually turned colorless, which proves that the cells are actively transporting the dye out of the cytoplasm. When the yeast cells are dead, they stayed red because active transport cannot occur in dead cells. Possible sources of error were the amount of Congo red dye, the amount of yeast cells, and if tall the yeast cells were properly hydrated.

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