Membrane Function Lab Planning – Hour 4, Group 1

Consider the following questions to help you get started:

  • What variable will you be testing (independent variable)?
  • What variable(s) will you be measuring (dependent variable)?
  • What variables will you hold constant (constant variable)?
  • What evidence would confirm that the stain has crossed the membrane?
  • How will you be confident in the validity of your results?
  • What will you use as a standard of comparison (control group)?

Use the comment form below to discuss the plan for your experiment.

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10 Responses to Membrane Function Lab Planning – Hour 4, Group 1

  1. Annabelle N says:

    Ok independent variable- what are we changing?

    Ok first of all what do we want to do for our experiment?

  2. Ben C. says:

    We will be testing the yeast`s ability to transfer the dye Congo Red in and out of the yeast cell through passive transport, and active transport. We will be measuring the amount of red dye that resides in the yeast after a period of maybe a few minutes. We will use the same microscope, same amount of dye, and the same type of yeast throughout the experiment. If the yeast turns into a red color then we will know that the dye has crossed its membrane. Our confidence will be affirmed if some red dye is moved out and some remains, this will prove passive transport is working, as well as active (for alive cells only). We will be using normal yeast as comparison (undid and unaltered).

  3. Annabelle N says:

    Independent variable is the amount of dye inside the yeast cell
    So our dependent variable is time

  4. Megan C says:

    Will we do the experiment more than once to be sure of its accuracy?

  5. Deepak D. says:

    We will test if the yeast will be able to transfer congo red dye in and out of the yeast cell. We will be measuring amount of red dye. We will use the same yeast,microscope, and same dye. If it turns red that means the dye has crossed the membrane. We will be confident if we do it a few more times and get the same result. We will be comparing it to other yeast.

  6. Annabelle N says:

    Yes @Megan

  7. Ben C. says:

    Yea, we will do it at least twice @Megan C.

  8. Annabelle N and Megan C says:

    CONCLUSION: we affirm the claim that yeast cells actively transport the dye out. We observed that the live, healthy cells became clear after some time (as they were previously red). The unhealthy cells (for the most part) stayed red. This is because unhealthy cells cannot actively transport molecules from their cytoplasm. Some possible sources of error are our counting/estimation skills or the amounts of dye and yeast culture that we used.

  9. Ben C. says:

    Here is a summary of our procedure: Our first was to set up the slides with active yeast cell`s, then we put Congo red onto the yeast cells. Then we put the cells under the microscope and examined and recorded notes. Then we repeated this process with dead cells.

  10. Deepak D says:

    I agree Ben since half of that was my words.

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