Membrane Function Lab Planning – Hour 1, Group 8

Consider the following questions to help you get started:

  • What variable will you be testing (independent variable)?
  • What variable(s) will you be measuring (dependent variable)?
  • What variables will you hold constant (constant variable)?
  • What evidence would confirm that the stain has crossed the membrane?
  • How will you be confident in the validity of your results?
  • What will you use as a standard of comparison (control group)?

Use the comment form below to discuss the plan for your experiment.

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10 Responses to Membrane Function Lab Planning – Hour 1, Group 8

  1. Aidan D says:

    So, here are my ideas for the variables(independent, dependent, and constant.):

    Independent Variable- the yeast cell’s health (dead or alive)
    Dependent Variable- the yeast cell’s color
    Constant(s)-the type of yeast cell, the amount of stain, the type of stain (Congo red).

  2. Aidan D says:

    My ideas on possible evidence to confirm the stain crossing the cell membrane:

    – If the yeast cell is dead, then the yeast cell’s cytoplasm changes color from white to red.
    – If the yeast cell is alive, then the yeast cell’s cytoplasm will turn red, then gradually change back to white.

    • Dylan Barker says:

      I would also say that if we see red dye specs after we remove the excess dye, then the stain has crossed the membrane.

  3. Aidan D says:

    My ideas for the last two:

    -The validity of our results will be backed by evidence from our observations and data gathered from the lab. The evidence should back up our claim, which confidently shows the validity of our results.

    -The control group would be the type of each material we are using in the experiment.

  4. Aidan D says:

    Please respond with any kind of agreement and/or revisions to the variables, evidence, validity, and control group.

  5. Aidan D. says:

    Conclusion(Aidan D.):
    Based on our evidence, the stain was actively transported out of the living yeast cells, but most of the dead yeast cells did not, or were not able to do that. Our evidence shows that among the living cells, no Congo red or red cells were found, but most of the dead cells were red. The reasoning behind this is that dead/unhealthy cells are unable to use active transport, but living cells can. Possible sources of error are unclean microscope slides (dry cheek cells from the previous experiment) and the amount of Congo red dye that was used.

  6. Dylan B. says:

    Procedure:
    1: First get a drop of live yeast calls and place them on the film.
    2: Next put a drop of Congo Red dye on the yeast calls and remove the excess dye. To remove the dye put some tap water on one side and a paper towel on the other side and slowly move the towel.
    3: Then put a cover slip on top of the yeast calls and put the film under the microscope and zoom and focus in on the cells.
    4: Finally, repeat the same process with dead cells.

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