Membrane Function Lab Planning – Hour 1, Group 4

Consider the following questions to help you get started:

  • What variable will you be testing (independent variable)?
  • What variable(s) will you be measuring (dependent variable)?
  • What variables will you hold constant (constant variable)?
  • What evidence would confirm that the stain has crossed the membrane?
  • How will you be confident in the validity of your results?
  • What will you use as a standard of comparison (control group)?

Use the comment form below to discuss the plan for your experiment.

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3 Responses to Membrane Function Lab Planning – Hour 1, Group 4

  1. Victoria R. says:

    Hey guys, I’m not sure if I’ll be able to get on again so here are my thoughts. I think the independent variable is the yeast and the dependent variable would be whether the Congo red crosses the membrane. Constants would be the type of dye and cell as well as the microscope.The evidence would be the color. Dead yeast cells can’t use active transport and the cell will remain red from the dye. To be confident in the validity of our results, we could do two trials for both the healthy and dead yeast cells. I do not have any ideas about the control group. Our procedure could involve gathering the materials, putting the yeast cells on the slide with the Congo red, then observing them under the microscope to look for any color changes. If you guys have any other thoughts or suggestions let me know. I’ll try to check this later but if not we can discuss at the beginning of class.

  2. Mae K says:

    I agree with you and the lab plan. Since it is like the cheek cell lab, we should be able to get everything done and answered fairly quickly but it might take longer because it’s with yeast cells. It could also take longer because there are active cells and we can’t get as many observations from the ones that are alive than the ones that aren’t. As of right now, I don’t know what we will do for the control group but if we talk tomorrow before class, we can figure one out that we all agree on.

  3. Ben B says:

    This lab should be fairly easy, like you said, because we have already done the cheek cell lab. The dead yeast cells will stay red, because they are dead and cannot preform any kind of transport; then the live cells will pump the red dye out, because they are alive and can transport small things in or out.

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